US 7,556,937 B2
	Method for producing ergosta-5,7-dienol and/or biosynthetic intermediate and/or secondary products thereof in transgenic organisms
Andreas Künkel, Neustadt (Germany); Markus Veen, Berlin (Germany); and Christine Lang, Berlin (Germany)
Assigned to OrganoBalance GmbH, Berlin (Germany)
Appl. No. 10/549,871 PCT Filed Mar. 12, 2004, PCT No. PCT/EP2004/002582 § 371(c)(1), (2), (4) Date Sep. 16, 2005, PCT Pub. No. WO2004/083407, PCT Pub. Date Sep. 30, 2004.
Claims priority of application No. 103 12 314 (DE), filed on Mar. 19, 2003.
Prior Publication US 2006/0269986 A1, Nov. 30, 2006
Int. Cl. C12P 33/00 (2006.01); C12N 9/02 (2006.01)

U.S. Cl. 435—52  [435/189]

14 Claims

1. A method for the production of ergosta-5,7-dienol comprising culturing a genetically modified yeast organism, wherein the genetic modification reduces the Δ22-desaturase activity consisting of the enzymatic activity of Δ22-desaturase having the amino acid sequence of SEQ ID.NO: 2 and increases the HMG-CoA reductase activity consisting of the enzymatic activity of HMG-CoA reductase having the amino acid sequence of SEQ ID.NO: 4 and increases squalene epoxidase activity consisting of the enzymatic activity of squalene epoxidase having the amino acid sequence of SEQ ID.NO: 8 in comparison with the wild type.