US 7,393,683 B2
	Mammalian host cell modified by RNAi to inhibit α 1,6-fucosyltransferase
Yutaka Kanda, Machida (Japan); Mitsuo Satoh, Machida (Japan); Kazuyasu Nakamura, Machida (Japan); Kazuhisa Uchida, Machida (Japan); Toyohide Shinkawa, Machida (Japan); Naoko Yamane, Machida (Japan); Emi Hosaka, Machida (Japan); Kazuya Yamano, Machida (Japan); Motoo Yamasaki, Machida (Japan); and Nobuo Hanai, Machida (Japan)
Assigned to Kyowa Hakko Kogyo Co., Ltd., Tokyo (Japan)
Filed on Nov. 28, 2005, as Appl. No. 11/287,324.
Application 11/287324 is a division of application No. 11/131212, filed on May 18, 2005.
Application 11/131212 is a division of application No. 09/971773, filed on Oct. 09, 2001, granted, now 6,946,292.
Claims priority of provisional application 60/268916, filed on Feb. 16, 2001.
Claims priority of application No. 2000-308526 (JP), filed on Oct. 06, 2000; and application No. PCT/JP01/08804 (JP), filed on Oct. 05, 2001.
Prior Publication US 2006/0078990 A1, Apr. 13, 2006
Int. Cl. C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12N 9/88 (2006.01); G01N 33/53 (2006.01); C12P 21/04 (2006.01); A01K 67/00 (2006.01); A01K 67/033 (2006.01)

U.S. Cl. 435—325  [435/375; 435/232; 435/7.8; 435/70.1; 800/8]

6 Claims

1. An isolated mammalian host cell in which the expression of the mRNA of α1,6-fucosyltransferase is inhibited by RNA interference, wherein when a gene encoding an antibody molecule is introduced into said host cell and expressed, said host cell produces an antibody composition comprising the encoded antibody molecule which includes an Fc region comprising complex N-glycoside-linked sugar chains bound to the Fc region, said N-glycoside-linked sugar chains having reduced levels of fucose bound to N-acetylglucosamine at the reducing end, thereby producing an antibody with increased antibody-dependent cellular cytotoxicity.