| US 7,393,668 B2 | ||
| Method of collecting highly pure polyhydroxyalkanoate from microbial cells | ||
| Yoshifumi Yanagita, Akashi (Japan); Noriko Ogawa, Kobe (Japan); Yasuyoshi Ueda, Himeji (Japan); Fumio Osakada, Okayama (Japan); and Keiji Matsumoto, Nishinomiya (Japan) | ||
| Assigned to Kaneka Corporation, Osaka-shi (Japan) | ||
| Appl. No. 10/541,389 PCT Filed Jan. 20, 2004, PCT No. PCT/JP2004/000416 § 371(c)(1), (2), (4) Date Jul. 01, 2005, PCT Pub. No. WO2004/065608, PCT Pub. Date Aug. 05, 2004. |
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| Claims priority of application No. 2003-011099 (JP), filed on Jan. 20, 2003. | ||
| Prior Publication US 2006/0084161 A1, Apr. 20, 2006 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12P 7/62 (2006.01); C12N 9/00 (2006.01) | ||
| U.S. Cl. 435—135 [435/183] | 20 Claims |
| 1. A method for recovering a polyhydroxyalkanoate from a polyhydroxyalkanoate-containing microbial cell
which comprises the following steps (a) and (b);
(a) a step comprising adding an alkali to an aqueous suspension of the polyhydroxyalkanoate-containing microbial cell while
stirring and carrying out a physical disruption treatment to disrupt the cell, solubilizing or emulsifying cell substances
other than the polyhydroxyalkanoate in said cell, and then separating the polyhydroxyalkanoate from the aqueous suspension,
and
(b) a step comprising treating the separated polyhydroxyalkanoate with an enzyme and/or a surfactant to solubilize impurities
adhering to the polyhydroxyalkanoate or to solubilize them after decomposing, and then washing the polyhydroxyalkanoate with
a hydrophilic solvent and/or water.
|