US 7,553,652 B2
	Process for the preparation of (S)- or (R)-3,3,3-trifluoro-2-hydroxy-2-methylpropionic acid
Walter Brieden, Glis (Switzerland); Andrew Naughton, Visp (Switzerland); Karen Robins, Visp (Switzerland); Nicholas Shaw, Visp (Switzerland); Andreas Tinschert, Brig (Switzerland); and Thomas Zimmermann, Naters (Switzerland)
Assigned to Lonza AG, Basel (Switzerland)
Filed on Aug. 10, 2005, as Appl. No. 11/200,182.
Application 11/200182 is a division of application No. 10/086082, filed on Feb. 28, 2002, granted, now 7,074,606.
Application 10/086082 is a division of application No. 09/214679, granted, now 6,773,910, previously published as PCT/EP97/03670, filed on Jul. 10, 1997.
Claims priority of application No. 1723/96 (CH), filed on Jul. 10, 1996; and application No. 500/97 (CH), filed on Mar. 03, 1997.
Prior Publication US 2006/0177919 A1, Aug. 10, 2006
Int. Cl. C12N 9/80 (2006.01)

U.S. Cl. 435—228

17 Claims

1. An isolated polypeptide having amidohydrolase activity capable of hydrolysing 3,3,3-trifluoro-2-hydroxy-2-methyl propionamide, in the form of the racemate or of an optically active isomer thereof, said polypeptide being encoded by a nucleic acid sequence which hybridizes under stringent conditions to the nucleotide sequence of SEQ ID NO:1, wherein said stringent hybridization conditions include hybridization at temperatures of between 60° C. and 70° C. and a salt content of 0.5 to 1.5M.