| US 7,553,627 B2 | ||
| Process for high throughput DNA methylation analysis | ||
| Peter W. Laird, South Pasadena, Calif. (US); Cindy A. Carroll, Arcadia, Calif. (US); and Kathleen D. Danenberg, Altadena, Calif. (US) | ||
| Assigned to University of Southern California, USC Stevens Center for Innovation, Los Angeles, Calif. (US) | ||
| Filed on Sep. 08, 2006, as Appl. No. 11/518,353. | ||
| Application 11/518353 is a continuation of application No. 10/016505, filed on Dec. 10, 2001, granted, now 7,112,404. | ||
| Application 10/016505 is a continuation of application No. 09/311912, filed on May 14, 1999, granted, now 6,331,393. | ||
| Prior Publication US 2007/0212699 A1, Sep. 13, 2007 | ||
| Int. Cl. C07H 21/04 (2006.01); C12Q 1/68 (2006.01) | ||
| U.S. Cl. 435—6 [435/91.1; 435/91.2; 435/91.21; 536/23.1; 536/24.31; 536/24.33] | 19 Claims |
| 1. A method for detecting cytosine methylation and methylated CpG islands within a genomic sample of DNA comprising:
(a) contacting a genomic sample of DNA with a modifying agent that modifies unmethylated cytosine to produce a converted nucleic
acid;
(b) amplifying the converted nucleic acid by means of oligonucleotide primers in the presence of a methylated CpG-specific
oligonucleotide probe and a non-methylated CpG-specific probe, wherein one or a plurality of the oligonucleotide primers and
the CpG-specific probes are capable of distinguishing between unmethylated and methylated nucleic acid; and
(c) detecting, in real time during the amplification, the methylated nucleic acid based on amplification-mediated displacement
of the probes, wherein quantifying methylation is afforded.
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