US 11,681,135 B2
Structured illumination microscopic imaging system
Daxi Xiong, Suzhou (CN); Xibin Yang, Suzhou (CN); and Peipei Wang, Suzhou (CN)
Assigned to Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou (CN)
Appl. No. 16/607,371
Filed by Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou (CN)
PCT Filed Jun. 20, 2017, PCT No. PCT/CN2017/089163
§ 371(c)(1), (2) Date Oct. 23, 2019,
PCT Pub. No. WO2018/205357, PCT Pub. Date Nov. 15, 2018.
Claims priority of application No. 201710334448.0 (CN), filed on May 12, 2017.
Prior Publication US 2020/0142171 A1, May 7, 2020
Int. Cl. G02B 21/06 (2006.01); G02B 21/00 (2006.01); G02B 5/08 (2006.01); G02B 27/09 (2006.01)
CPC G02B 21/06 (2013.01) [G02B 21/006 (2013.01); G02B 5/08 (2013.01); G02B 27/09 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A structured illumination microscopic imaging system, comprising:
a structured illumination source;
a beam shaping lens, an excitation optical filter and a dichroic mirror, sequentially provided on an emission light path of the structured illumination source; wherein, the beam shaping lens comprises more than one lens;
an objective lens and a sample, sequentially provided on a first optical path of the dichroic mirror; and
an emission optical filter, a tube lens, and a detector, sequentially provided on a second optical path of the dichroic mirror;
wherein the structured illumination source comprises a micro-sized illuminating light source configured to produce structured light having bright and dark fringes;
wherein the micro-sized illuminating light source comprises a substrate and an array of light emitting units arranged on the substrate; wherein a size of each light emitting unit has a square area in a range of 1 μm×1 μm to 100 μm×100 μm;
wherein each of the light emitting units in the array of light emitting units is independently controllable for obtaining structural light fringes;
wherein a duty ratio is a ratio of the bright and dark fringes, and the duty ratio is one of 1:1, 1:2, or 1:3; and
wherein a spacing between two adjacent light emitting units is determined by a shape of the light emitting unit, a number of lenses included in the beam shaping lens, a focal length of the beam shaping lens, a magnification of the objective lens, and a focal length of the objective lens, causing a spacing between adjacent images on a surface of the sample is less than half of a wavelength of light emitted from the light emitting units.