US 11,680,890 B2
Real-time mitochondrial dimension measurements
Yael Yaniv, Haifa (IL); Joseph M. Leichner, Nof Ayalon (IL); and Evgene Konyukhov, Nesher (IL)
Assigned to TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED, Haifa (IL)
Appl. No. 17/47,814
Filed by TECHNION RESEARCH & DEVELOPMENT FOUNDATION LIMITED, Haifa (IL)
PCT Filed Apr. 17, 2019, PCT No. PCT/IL2019/050444
§ 371(c)(1), (2) Date Oct. 15, 2020,
PCT Pub. No. WO2019/202599, PCT Pub. Date Oct. 24, 2019.
Claims priority of provisional application 62/658,662, filed on Apr. 17, 2018.
Prior Publication US 2021/0110546 A1, Apr. 15, 2021
Int. Cl. G06T 7/62 (2017.01); G06V 20/69 (2022.01); G06V 10/42 (2022.01); G01N 15/14 (2006.01); G06T 7/00 (2017.01)
CPC G01N 15/1475 (2013.01) [G06T 7/0016 (2013.01); G06T 7/62 (2017.01); G06V 10/431 (2022.01); G06V 20/695 (2022.01); G06T 2207/10016 (2013.01); G06T 2207/20056 (2013.01); G06T 2207/30004 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method comprising:
receiving a live video sequence comprising two or more image frames depicting a live mammalian cell;
defining, in real-time, within said cell, at least one longitudinal line along a length dimension of said cell, and at least one transverse line along a width dimension of said cell;
calculating, in real-time, with respect to each image frame in said video sequence, a fast Fourier transform (FFT) with respect to each of said at least one longitudinal and transverse lines;
detecting peaks in each of said calculated FFTs; and
determining, for every image frame in the video sequence, in real-time, with respect to said cell, one or more temporal changes in at least one of sarcomere length, mitochondrial length, and mitochondrial width, based, at least in part, on said detected peaks, thereby detecting mitochondrial contraction-relaxation cycles in said live mammalian cell.