US 11,680,281 B2
Biosynthetic production of steviol glycosides and processes therefore
Guohong Mao, Burlington, MA (US); Jacob Edward Vick, Cambridge, MA (US); Michael Batten, Westford, MA (US); David Byun, Bedford, MA (US); Yang Luo, Wuxi Jiangsu (CN); Yilin Wu, Wuxi Jiangsu (CN); Beihua Zhang, Wuxi Jiangsu (CN); and Xiaodan Yu, Lexington, MA (US)
Assigned to Conagen Inc., Bedford, MA (US)
Filed by Conagen Inc., Bedford, MA (US)
Filed on Jun. 18, 2020, as Appl. No. 16/904,797.
Application 16/904,797 is a continuation of application No. 16/382,280, filed on Apr. 12, 2019, granted, now 10,724,062.
Application 16/382,280 is a continuation of application No. PCT/US2017/056457, filed on Oct. 13, 2017.
Claims priority of provisional application 62/555,809, filed on Sep. 8, 2017.
Claims priority of provisional application 62/408,179, filed on Oct. 14, 2016.
Prior Publication US 2021/0002685 A1, Jan. 7, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/18 (2006.01); C12P 19/56 (2006.01); A23L 27/30 (2016.01); A23L 2/60 (2006.01); C07H 15/24 (2006.01); C12P 19/44 (2006.01); A21D 13/062 (2017.01); A23G 4/10 (2006.01); C07H 1/00 (2006.01); C07H 15/256 (2006.01); C12N 9/10 (2006.01); C12N 9/42 (2006.01); C12N 15/74 (2006.01); C12N 15/81 (2006.01); C12N 15/82 (2006.01); A23K 20/163 (2016.01)
CPC C12P 19/56 (2013.01) [A21D 13/062 (2013.01); A23G 4/10 (2013.01); A23L 2/60 (2013.01); A23L 27/36 (2016.08); C07H 1/00 (2013.01); C07H 15/24 (2013.01); C07H 15/256 (2013.01); C12N 9/1062 (2013.01); C12N 9/1081 (2013.01); C12N 9/2445 (2013.01); C12N 15/74 (2013.01); C12N 15/81 (2013.01); C12N 15/8243 (2013.01); C12N 15/8245 (2013.01); C12P 19/18 (2013.01); C12P 19/44 (2013.01); A23K 20/163 (2016.05); A23V 2002/00 (2013.01); C12Y 204/01013 (2013.01); C12Y 204/01028 (2013.01); C12Y 302/01021 (2013.01)] 8 Claims
 
1. A method of producing rebaudioside M, the method comprising:
providing a reaction mixture comprising:
(a) rebaudioside D4 having the structure:

OG Complex Work Unit Chemistry
(b) uridine diphosphate-glucose (UDP-glucose); and
(c) a UDP-glycosyltransferase (UGT) comprising an amino acid sequence having at least 90% identity to SEQ ID NO: 1 or SEQ ID NO: 3; and
incubating the reaction mixture for a sufficient time to produce rebaudioside M.