CPC C12P 19/56 (2013.01) [A21D 13/062 (2013.01); A23G 4/10 (2013.01); A23L 2/60 (2013.01); A23L 27/36 (2016.08); C07H 1/00 (2013.01); C07H 15/24 (2013.01); C07H 15/256 (2013.01); C12N 9/1062 (2013.01); C12N 9/1081 (2013.01); C12N 9/2445 (2013.01); C12N 15/74 (2013.01); C12N 15/81 (2013.01); C12N 15/8243 (2013.01); C12N 15/8245 (2013.01); C12P 19/18 (2013.01); C12P 19/44 (2013.01); A23K 20/163 (2016.05); A23V 2002/00 (2013.01); C12Y 204/01013 (2013.01); C12Y 204/01028 (2013.01); C12Y 302/01021 (2013.01)] | 8 Claims |
1. A method of producing rebaudioside M, the method comprising:
providing a reaction mixture comprising:
(a) rebaudioside D4 having the structure:
(b) uridine diphosphate-glucose (UDP-glucose); and
(c) a UDP-glycosyltransferase (UGT) comprising an amino acid sequence having at least 90% identity to SEQ ID NO: 1 or SEQ ID NO: 3; and
incubating the reaction mixture for a sufficient time to produce rebaudioside M.
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