CPC C12N 15/81 (2013.01) [C12N 15/1037 (2013.01); C40B 40/08 (2013.01); C12N 2800/102 (2013.01)] | 16 Claims |
1. A polynucleotide construct comprising the nucleotide sequence of SEQ ID NO:1, the polynucleotide construct comprising the structure of Expression (I), wherein Expression (I) comprises:
5′-A-B-C-D-E-F-3′ (I); wherein,
A represents the promoter;
B represents the multiple cloning site region;
C represents the CH1 fragment of the human IgG heavy chain constant region;
D represents the linker sequence, wherein the linker is selected from the group consisting of (Gly4Ser)3 and (GlySer)5;
E represents the yeast surface positioning sequence; and
F represents none or the tag sequence, wherein the tag sequence is selected from the group consisting of c-Myc, V5, hexahistidine, glutathione-S-transferase, streptavidin, biotin, hemagglutinin, Flag tag and E tag.
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