CPC C12N 15/635 (2013.01) [C12N 15/85 (2013.01); C12N 2800/10 (2013.01); C12N 2800/22 (2013.01); C12N 2830/001 (2013.01); C12N 2830/003 (2013.01)] | 20 Claims |
1. A nucleic acid sequence, comprising at least 2 copies of Tet operator (TetO) sequences capable of binding to a reverse tetracycline controlled transactivator (rtTA) regulatable by tetracycline or a derivative thereof, 1 copy of a minimal promoter sequence containing a TATA box sequence, and at least 1 copy of a Cumate operator (CuO) sequence capable of binding to a transcription repressor cysteine metabolism repressor (CymR) regulatable by cumate, wherein the CuO-sequence is downstream of the 3′ end of the TATA box sequence, and is 30 bp to 50 bp apart from the TATA box.
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