	US 11,680,251 B2
	Method for assembling prenyl groups onto cyclic dipeptides
Wenli Li, Shandong (CN); Tingting Yao, Shandong (CN); Jing Liu, Shandong (CN); Huayue Li, Shandong (CN); and Tong Li, Shandong (CN)
Assigned to OCEAN UNIVERSITY OF CHINA, Shandong (CN)
Filed by OCEAN UNIVERSITY OF CHINA, Shandong (CN)
Filed on Jan. 31, 2022, as Appl. No. 17/589,494.
Application 17/589,494 is a continuation of application No. 17/042,942, granted, now 11,279,918, previously published as PCT/CN2019/097372, filed on Jul. 23, 2019.
Claims priority of application No. 201810831718.3 (CN), filed on Jul. 26, 2018.
Prior Publication US 2022/0154152 A1, May 19, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/10 (2006.01)

CPC C12N 9/1085 (2013.01) [C12Y 205/01 (2013.01)]

3 Claims

1. A method for assembling prenyl groups onto cyclic dipeptides, comprising steps of:

encoding a preyltransferase with a recombinant plasmid, wherein the recombinant plasmid comprises a gene encoding the preyltransferase; and

using the prenyltransferase for assembling the prenyl groups to Trp-containing cyclic dipeptides;

wherein the prenyltransferase comprises the amino acid sequence of SEQ ID NO:1.