	US 11,680,248 B2
	Recombinant herpes simplex virus and use thereof
Chenghao Huang, Xiamen (CN); Yong Luo, Xiamen (CN); Quan Yuan, Xiamen (CN); Jun Zhang, Xiamen (CN); and Ningshao Xia, Xiamen (CN)
Assigned to XIAMEN UNIVERSITY, Xiamen (CN); and YANG SHENG TANG COMPANY, LTD., Haikou (CN)
Appl. No. 16/491,699
Filed by Xiamen University, Xiamen (CN); and Yang Sheng Tang Company, LTD., Haikou (CN)
PCT Filed Feb. 28, 2018, PCT No. PCT/CN2018/077518 § 371(c)(1), (2) Date Sep. 6, 2019, PCT Pub. No. WO2018/161825, PCT Pub. Date Sep. 13, 2018.
Claims priority of application No. 201710136638.1 (CN), filed on Mar. 9, 2017; and application No. 201710301464.X (CN), filed on May 2, 2017.
Prior Publication US 2020/0071679 A1, Mar. 5, 2020
Int. Cl. C12N 7/00 (2006.01); A61P 35/00 (2006.01); A61K 35/763 (2015.01); C07K 14/005 (2006.01); C12N 15/86 (2006.01)

CPC C12N 7/00 (2013.01) [A61K 35/763 (2013.01); A61P 35/00 (2018.01); C07K 14/005 (2013.01); C12N 15/86 (2013.01); C12N 2710/16621 (2013.01); C12N 2710/16622 (2013.01); C12N 2710/16643 (2013.01); C12N 2710/16651 (2013.01)]

19 Claims

1. A recombinant herpes simplex virus (HSV), which does not express a functional ICP0 protein and ICP34.5 protein; but is capable of expressing a functional UL43 protein, a functional UL41 protein, a functional UL48 protein, or any combination thereof; and wherein the genome of the recombinant HSV comprises the following modifications:

two copies of the ICP0 gene each independently comprising a loss-of-function mutation or which is deleted or substituted with an exogenous nucleotide sequence;

two copies of the ICP34.5 gene each independently comprising a loss-of-function mutation or which is deleted or substituted with an exogenous nucleotide sequence; and

wherein the genome of the recombinant HSV further comprises a modification in which a native promoter of one or more HSV genes is substituted with a tumor-specific promoter.