US 11,680,239 B2
Filter for mammalian cell culture perfusion and clarification with hydrophobic hollow fiber
Michael Bransby, Altadena, CA (US); Derek Carroll, Los Angeles, CA (US); and Philip Yuen, Long Beach, CA (US)
Assigned to Repligen Corporation, Waltham, MA (US)
Filed by Repligen Corporation, Waltham, MA (US)
Filed on Dec. 30, 2019, as Appl. No. 16/730,516.
Claims priority of provisional application 62/846,934, filed on May 13, 2019.
Claims priority of provisional application 62/786,884, filed on Dec. 31, 2018.
Prior Publication US 2020/0208092 A1, Jul. 2, 2020
Int. Cl. C12M 1/12 (2006.01); C12M 3/00 (2006.01); B01D 69/08 (2006.01); B01D 63/04 (2006.01); C12M 1/00 (2006.01); B01D 71/26 (2006.01)
CPC C12M 25/10 (2013.01) [B01D 63/04 (2013.01); B01D 69/081 (2013.01); B01D 71/26 (2013.01); C12M 21/08 (2013.01); C12M 29/10 (2013.01); B01D 2239/0428 (2013.01); B01D 2239/1216 (2013.01); B01D 2239/1225 (2013.01)] 40 Claims
OG exemplary drawing
 
1. A filter housing comprising:
an inlet;
a retentate outlet;
a permeate outlet;
at least one pre-wetted hydrophobic hollow fiber filter extending between the inlet and the retentate outlet, thereby fluidly separating the permeate outlet from the retentate outlet and the inlet; and
a potting material connecting the at least one hydrophobic hollow fiber filter to the filter housing and sealing at least one of the inlet and the retentate outlet from the permeate outlet,
wherein the pre-wetted hydrophobic hollow fiber filter consists of hydrophobic polymers and lacks hydrophilic polymers, and
wherein the filter is contained in an aqueous solution within the filter housing.