US 11,679,382 B2
Nucleic acid extraction system
Chuli Yi, Wuhan (CN)
Assigned to WUHAN EDEBIO TECHNOLOGY LLC., Hubei (CN)
Appl. No. 16/971,665
Filed by WUHAN EDEBIO TECHNOLOGY LLC., Hubei (CN)
PCT Filed Mar. 8, 2019, PCT No. PCT/CN2019/077403
§ 371(c)(1), (2) Date Aug. 20, 2020,
PCT Pub. No. WO2019/174524, PCT Pub. Date Sep. 19, 2019.
Claims priority of application No. 201810206072.X (CN), filed on Mar. 13, 2018.
Prior Publication US 2020/0391198 A1, Dec. 17, 2020
Int. Cl. G01N 35/10 (2006.01); B01L 3/02 (2006.01); B01L 3/00 (2006.01); B01L 9/00 (2006.01); C12N 15/10 (2006.01); B01F 27/806 (2022.01)
CPC B01L 3/021 (2013.01) [B01F 27/806 (2022.01); B01L 3/52 (2013.01); B01L 3/565 (2013.01); B01L 3/567 (2013.01); B01L 9/523 (2013.01); C12N 15/1017 (2013.01); G01N 35/1002 (2013.01); B01L 2200/16 (2013.01); B01L 2300/042 (2013.01); B01L 2300/0618 (2013.01); B01L 2300/14 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/082 (2013.01); G01N 35/1009 (2013.01); G01N 35/1011 (2013.01); G01N 35/1074 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A nucleic acid extraction system, comprising an installation frame, a nucleic acid extraction plate, a pipetting device and a positive pressure device, wherein the pipetting device and the positive pressure device are arranged side by side,
the pipetting device comprises an infusion set, an infusion set mounting frame, an infusion set drive unit and a plurality of infusion units, the infusion set comprises sealed chambers which correspond to the plurality of infusion units in one-to-one correspondence, the sealed chambers are arranged side by side, a needle is provided on the bottom of each sealed chamber, each needle is in communication with the corresponding sealed chamber, the infusion set is arranged on the bottom of the infusion set mounting frame, and the infusion set drive unit is fixed on the installation frame and is configured to drive the infusion set to move up and down through the infusion set mounting frame;
each infusion unit comprises a reagent supplier and a reagent flow controller, and in each infusion unit, a liquid outlet of the reagent supplier is in communication with a liquid inlet of the reagent flow controller, and a liquid outlet of the reagent flow controller is in communication with the corresponding sealed chamber;
the positive pressure device comprises a plurality of plugs, a plurality of corresponding air pipes, a positive pressure provider and a positive pressure drive device, each of the plugs is provided with an air pipe communicating hole, one end of a corresponding air pipe hermetically communicates with the air pipe communicating hole and another end of the corresponding air pipe hermetically communicates with an air outlet of the positive pressure provider, and the positive pressure drive device is fixed on the installation frame and is configured to drive the positive pressure provider to move up and down; and
a plurality of nucleic acid extraction columns are provided on the nucleic acid extraction plate, the plurality of nucleic acid extraction columns are correspondingly arranged below the needles or the plugs, a liquid inlet of each nucleic acid extraction column is provided with a sealing gasket which is in a sealing fit with the corresponding plug, and the nucleic acid extraction column is detachably and hermetically connected with the corresponding plug.