CPC B01D 15/3833 (2013.01) [B01D 15/40 (2013.01)] | 15 Claims |
1. A method for the chiral separation of stereoisomers of α-tocopherol or α-tocopheryl acetate in a sample, the method comprising:
a. providing a sample comprising at least one stereoisomer of α-tocopherol or α-tocopheryl acetate;
b. contacting said sample with a column chromatography device comprising a first column having an interior portion at least partially filled with an immobilized stationary phase, wherein the immobilized stationary phase comprises amylose tris(3,5-dimethylphenylcarbamate), cellulose tris(3,5-dimethylphenylcarbamate), or cellulose tris(3-chloro-4-methylphenylcarbamate);
c. flowing a mobile phase through the immobilized stationary phase for a period of time, the mobile phase comprising carbon dioxide and a co-solvent which is a combination of acetonitrile and water; and
d. eluting the at least one stereoisomer of α-tocopherol or α-tocopheryl acetate from the immobilized stationary phase in the mobile phase; and
e. detecting the presence of the at least one stereoisomer of α-tocopherol or α-tocopheryl acetate using a detector.
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