US 11,679,341 B2
Differentiation of natural vitamin E from synthetic vitamin E and quantification of tocopherols by supercritical fluid chromatography
Jinchuan Yang, Hopkinton, MA (US); and Paul Rainville, Princeton, MA (US)
Assigned to Waters Technologies Corporation, Milford, MA (US)
Filed by Waters Technologies Corporation, Milford, MA (US)
Filed on Apr. 29, 2021, as Appl. No. 17/244,451.
Claims priority of provisional application 63/017,994, filed on Apr. 30, 2020.
Prior Publication US 2021/0339167 A1, Nov. 4, 2021
Int. Cl. B01D 15/38 (2006.01); B01D 15/40 (2006.01)
CPC B01D 15/3833 (2013.01) [B01D 15/40 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for the chiral separation of stereoisomers of α-tocopherol or α-tocopheryl acetate in a sample, the method comprising:
a. providing a sample comprising at least one stereoisomer of α-tocopherol or α-tocopheryl acetate;
b. contacting said sample with a column chromatography device comprising a first column having an interior portion at least partially filled with an immobilized stationary phase, wherein the immobilized stationary phase comprises amylose tris(3,5-dimethylphenylcarbamate), cellulose tris(3,5-dimethylphenylcarbamate), or cellulose tris(3-chloro-4-methylphenylcarbamate);
c. flowing a mobile phase through the immobilized stationary phase for a period of time, the mobile phase comprising carbon dioxide and a co-solvent which is a combination of acetonitrile and water; and
d. eluting the at least one stereoisomer of α-tocopherol or α-tocopheryl acetate from the immobilized stationary phase in the mobile phase; and
e. detecting the presence of the at least one stereoisomer of α-tocopherol or α-tocopheryl acetate using a detector.