US 11,679,133 B2
Methods for differentiating and purifying pancreatic endocrine cells
Kfir Molakandov, Yahud (IL); Neta Lavon, Nes-Ziona (IL); Avital Beck, Rehovot (IL); Michel Revel, Rehovot (IL); Ofer Elhanani, Tel-Aviv (IL); Yoav Soen, Rehovot (IL); Michael Walker, Rehovot (IL); and Arik Hasson, Kiryat-Ono (IL)
Assigned to Kadimastem Ltd., Nes Ziona (IL); and Yeda Research and Development Co. Ltd., Rehovot (IL)
Appl. No. 15/780,153
Filed by Kadimastem Ltd., Nes Ziona (IL); and Yeda Research and Development Co. Ltd., Rehovot (IL)
PCT Filed Nov. 28, 2016, PCT No. PCT/IL2016/051274
§ 371(c)(1), (2) Date May 30, 2018,
PCT Pub. No. WO2017/094001, PCT Pub. Date Jun. 8, 2017.
Claims priority of provisional application 62/366,651, filed on Jul. 26, 2016.
Claims priority of provisional application 62/260,669, filed on Nov. 30, 2015.
Prior Publication US 2018/0369290 A1, Dec. 27, 2018
Int. Cl. A61K 35/39 (2015.01); C12N 5/00 (2006.01); C12N 5/071 (2010.01); A61P 5/50 (2006.01); A61P 3/10 (2006.01)
CPC A61K 35/39 (2013.01) [A61P 3/10 (2018.01); A61P 5/50 (2018.01); C12N 5/0676 (2013.01); C12N 2500/25 (2013.01); C12N 2500/34 (2013.01); C12N 2500/38 (2013.01); C12N 2501/599 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01)] 6 Claims
 
1. A method of enriching for pancreatic endocrine cells expressing NKX6.1 and insulin comprising:
a) exposing an in vitro cell population comprising pancreatic endocrine cells expressing NKX6.1 and insulin having been ex-vivo differentiated from pluripotent stem cells to a ligand that binds CD49a;
b) exposing the in vitro cell population comprising pancreatic endocrine cells expressing NKX6.1 and insulin having been ex-vivo differentiated from pluripotent stem cells to a ligand that binds CD26;
c) selecting cells which bind said ligand that binds CD49a and do not bind said ligand that binds CD26; and subsequently
d) culturing said cells which bind said ligand that binds CD49a and do not bind said ligand that binds CD26, thereby enriching for pancreatic endocrine cells expressing NKX6.1 and insulin.