	US 7,550,567 B2
	Fibrinogen purification
Hubert Metzner, Marburg (Germany); Uwe Liebing, Coelbe (Germany); Annette Feussner, Marburg (Germany); Joerg Lemmer, Ebsdorfergrund (Germany); Stefan Schulte, Marburg (Germany); and Volker Gawantka, Lahntal (Germany)
Assigned to CSL Behring GmbH, Marburg (Germany)
Filed on Feb. 23, 2005, as Appl. No. 11/62,432.
Claims priority of application No. 10 2004 009 400 (DE), filed on Feb. 24, 2004.
Prior Publication US 2005/0197493 A1, Sep. 08, 2005
Int. Cl. C07K 14/75 (2006.01); C07K 1/16 (2006.01)

U.S. Cl. 530—382 [530/412; 530/415; 530/416; 530/417]

21 Claims

1. A process for purifying fibrinogen from a fibrinogen solution containing at least one contaminating protein, comprising:

subjecting the fibrinogen solution to negative chromatography using at least one of a cation exchanger, hydrophobic gel and dye gel, wherein the negative chromatography is performed using either column chromatography or batch chromatography, wherein interaction of the at least one contaminating protein with a stationary phase over which the fibrinogen solution passes is stronger than interaction of fibrinogen with the stationary phase, such that a majority of the fibrinogen is eluted from a column of the column chromatography, or remains in a supernatant of the batch chromatography, at the same time that the at least one contaminating protein is mainly bound to the stationary phase.