| US 7,550,148 B2 | ||
| Targeting of Herpes simplex virus to specific receptors | ||
| Guoying Zhou, Chicago, Ill. (US); and Bernard Roizman, Chicago, Ill. (US) | ||
| Assigned to The University of Chicago, Chicago, Ill. (US) | ||
| Filed on Aug. 30, 2005, as Appl. No. 11/215,636. | ||
| Application 11/215636 is a continuation in part of application No. 10/530774, previously published as PCT/US03/31598, filed on Oct. 06, 2003. | ||
| Claims priority of provisional application 60/416716, filed on Oct. 07, 2002. | ||
| Prior Publication US 2007/0190027 A1, Aug. 16, 2007 | ||
| Int. Cl. A01N 63/00 (2006.01); A61K 48/00 (2006.01); A61K 39/245 (2006.01); C12N 7/01 (2006.01) | ||
| U.S. Cl. 424—199.1 [424/93.2; 424/231.1; 435/235.1; 435/320.1] | 23 Claims |
| 1. A recombinant herpes simplex virus (HSV) particle having at least one protein on its surface, comprising:
(a) an altered viral surface protein, wherein the alteration reduces binding of the viral surface protein to a sulfated proteoglycan;
and
(b) an altered gD, wherein the alteration reduces binding of gD to one or more of its cellular receptors, said alteration
comprising
(i) a heterologous peptide ligand on the surface of the recombinant HSV particle forming a fusion protein with the altered
gD; and
(ii) a V34S amino acid substitution; wherein said recombinant HSV particle preferentially binds to cells expressing a binding partner to said heterologous peptide
ligand.
|