| US 7,387,880 B2 | ||
| Method for monitoring resting and activated platelets in unfixed blood samples | ||
| Timothy L. Wyant, Fremont, Calif. (US); Paul A. Raju, Union City, Calif. (US); and Aaron B. Kantor, San Carlos, Calif. (US) | ||
| Assigned to PPD Biomarker Discovery Sciences, LLC, Menlo Park, Calif. (US) | ||
| Filed on Mar. 07, 2005, as Appl. No. 11/75,024. | ||
| Application 11/075024 is a division of application No. 09/957624, filed on Sep. 20, 2001, granted, now 6,924,114. | ||
| Claims priority of provisional application 60/234065, filed on Sep. 20, 2000. | ||
| Prior Publication US 2005/0214877 A1, Sep. 29, 2005 | ||
| Int. Cl. G01N 33/53 (2006.01); G01N 1/00 (2006.01) | ||
| U.S. Cl. 435—7.2 [435/2; 435/7.1; 435/7.21; 435/374; 435/375; 436/517; 436/546; 436/18; 436/63; 436/69; 436/164; 436/166; 436/171; 436/172; 436/173; 436/176] | 22 Claims |
| 1. A method for measuring the amount of a platelet surface protein in a sample comprising platelets, comprising the steps
of:
(a) contacting the sample with a platelet stabilizing composition comprising an anticoagulant and at least one platelet activation
inhibitor;
(b) incubating the sample with a labeled compound having specific affinity for the platelet surface protein and a platelet
stimulating factor; and
(c) detecting labeled compound bound to platelets by cytometry, whereby the amount of the platelet surface protein is measured,
and wherein steps a), b), and c) are performed without fixation of the sample.
|