| US 7,547,819 B2 | ||
| Plant producing hyaluronic acid | ||
| Shigeo Shibatani, Otsu (Japan); Shuhei Misawa, Tsuruga (Japan); Izumi Ihara, Otsu (Japan); and Hiroaki Kitazawa, Otsu (Japan) | ||
| Assigned to Toyo Boseki Kabushiki Kaisha, Osaka-Shi (Japan) | ||
| Appl. No. 10/566,157 PCT Filed Jul. 30, 2004, PCT No. PCT/JP2004/011306 § 371(c)(1), (2), (4) Date Jan. 27, 2006, PCT Pub. No. WO2005/012529, PCT Pub. Date Feb. 10, 2005. |
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| Claims priority of application No. 2003-204896 (JP), filed on Jul. 31, 2003; and application No. 2004-089135 (JP), filed on Mar. 25, 2004. | ||
| Prior Publication US 2006/0168690 A1, Jul. 27, 2006 | ||
| Int. Cl. C12N 15/33 (2006.01); C12N 15/52 (2006.01); C12N 15/63 (2006.01); C12N 15/83 (2006.01); A01H 5/00 (2006.01) | ||
| U.S. Cl. 800—280 [800/288; 800/287; 800/298; 435/320.1; 435/468; 536/23.1; 536/23.72] | 11 Claims |
| 1. A method of producing hyaluronic acid comprising
(1) a step of transforming a plant cell using an expression recombinant vector comprising
(i) a DNA encoding hyaluronic acid synthase or
(ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase,
(2) a step of growing a transformant obtained by transformation of step (1), and
(3) a step of separating the hyaluronic acid produced by the transformant, wherein the hyaluronic acid synthase synthase is
derived from a chlorella virus.
|