| US 7,547,557 B2 | ||
| Directed-flow assay device | ||
| Ronald T. LaBorde, San Diego, Calif. (US); and Herbert S. Chow, San Diego, Calif. (US) | ||
| Assigned to Quantum Design, Inc., San Diego, Calif. (US) | ||
| Filed on Jun. 13, 2006, as Appl. No. 11/452,725. | ||
| Prior Publication US 2007/0287198 A1, Dec. 13, 2007 | ||
| Int. Cl. G01N 33/53 (2006.01) | ||
| U.S. Cl. 436—514 [436/518; 436/520; 436/523; 436/531; 436/535; 436/807; 436/808; 436/810; 435/287.1; 435/287.7; 435/805; 435/810; 435/4; 435/287.8; 435/287.9; 435/288.2; 435/288.4; 435/288.5; 422/50; 422/56; 422/58; 422/60; 422/61] | 5 Claims |
| 1. A lateral flow assay device for quantitative detection of target analytes in a sample, said device comprising:
an assay support member having a first end and a second end;
a porous analytical membrane mounted adjacent to and generatly parallel with said support member, said analytical membrane
having a first end and a second end and at least one capture region intermediate said first and second ends thereof, said
at least one capture region being configured to capture labeled analytes moving from said first end of said analytical membrane
toward said second end of said analytical membrane; and
a sample receiving port at one end of said support member for introduction into said device of the sample to be analyzed,
said sample receiving port comprising:
a fluid sealing material on said support member;
a channel layer positioned above said sealing material, said channel layer comprising an opening and a channel, said opening
providing fluid comunication with said channel, and said channel providing fluid communication with said porous analytical
membrane;
a hydrophilic material positioned over said channel layer, said hydrophilic material having an opening therein corresponding
to said opening in said channel layer; and
a gasket element positioned over said hydrophilic material and having an opening therein to allow fluid entry into the port,
said gasket providing a fluid seal for the assay device.
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