| US 7,547,526 B2 | ||
| Cell concentration and pathogen recovery | ||
| Michael R. Ladisch, West Lafayette, Ind. (US); Xingya Liu, West Lafayette, Ind. (US); Amanda C. Stewart, Kula, Hi. (US); Wan-Tzu Chen, Lafayette, Ind. (US); Nathan S. Mosier, West Lafayette, Ind. (US); Thomas Huang, West Lafayette, Ind. (US); Jeremiah Bwatwa, West Lafayette, Ind. (US); and Richard Hendrickson, West Lafayette, Ind. (US) | ||
| Assigned to Purdue Research Foundation, West Lafayette, Ind. (US) | ||
| Filed on Mar. 15, 2005, as Appl. No. 11/81,378. | ||
| Claims priority of provisional application 60/553235, filed on Mar. 15, 2004. | ||
| Prior Publication US 2005/0244943 A1, Nov. 03, 2005 | ||
| Int. Cl. C12Q 1/24 (2006.01) | ||
| U.S. Cl. 435—30 | 17 Claims |
| 1. A method of rapid concentration and recovery of viable microorganisms from a sample, the method comprising:
(a) providing a microorganism-containing sample, the sample comprising a liquid component having a first volume;
(b) providing a hollow fiber filter comprising at least one hollow fiber strand, wherein the hollow fiber filter comprises
fibers having a length between 1 cm and 25 cm;
(c) reducing the first volume of the liquid component by applying the sample to the hollow fiber filter, wherein the volume
is reduced by at least 1000 times;
(d) back flushing the microorganisms from the hollow fiber filter to remove the microorganisms from the hollow fiber filter
without vortexing and in a buffer having a second volume;
(e) delivering the second volume and the microorganisms from the hollow fiber filter to a biochip device; and
(f) detecting the presence of viable microorganisms using the biochip device.
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