| US 7,547,522 B2 | ||
| Method to enrich for α(1,3)-galactosyltransferase null pig cells | ||
| Robert J. Hawley, Wayland, Mass. (US) | ||
| Assigned to Immerge Biotherapeutics, Inc., Cambridge, Mass. (US) | ||
| Appl. No. 10/524,381 PCT Filed Aug. 13, 2003, PCT No. PCT/US03/25199 § 371(c)(1), (2), (4) Date Oct. 03, 2005, PCT Pub. No. WO2004/016742, PCT Pub. Date Feb. 26, 2004. |
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| Claims priority of provisional application 60/403405, filed on Aug. 14, 2002. | ||
| Prior Publication US 2006/0242722 A1, Oct. 26, 2006 | ||
| Int. Cl. G01N 33/53 (2006.01); G01N 33/567 (2006.01) | ||
| U.S. Cl. 435—7.21 [435/7.2; 435/7.1] | 15 Claims |
| 1. A method of selecting GGTA1 null cells comprising the steps of:
(a) obtaining a line of cells obtained from a GGTA1 heterozygous swine or swine fetus;
(b) enriching the cells for GGTA1 null cells by treating the cells with agents that specifically bind an α(1,3)-galactose
epitope and that deplete cells that express the epitope; and
(c) scanning the line for viable GGTA1 null cells.
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