| US 7,546,779 B2 | ||
| Vacuum assisted affinity chromatography device and method | ||
| Aldo M. Pitt, Wayland, Mass. (US); Gary LaBombard, Concord, N.H. (US); and Phillip Clark, Wakefield, Mass. (US) | ||
| Assigned to Millipore Corporation, Billerica, Mass. (US) | ||
| Filed on Jan. 26, 2007, as Appl. No. 11/698,663. | ||
| Application 11/698663 is a division of application No. 11/051047, filed on Feb. 04, 2005, granted, now 7,240,572. | ||
| Claims priority of provisional application 60/545671, filed on Feb. 18, 2004. | ||
| Prior Publication US 2007/0227975 A1, Oct. 04, 2007 | ||
| Int. Cl. G01N 1/00 (2006.01) | ||
| U.S. Cl. 73—863.21 | 8 Claims |
| 1. A method of purifying a sample, comprising:
providing a sample reservoir in fluid communication with a first filter unit comprising chromatography media positioned in
a manifold in communication with a vacuum source and in fluid communication with a centrifugal filter unit downstream of said
first filter unit;
introducing said sample into said sample reservoir;
drawing said sample through said sample reservoir into said first filter unit by application of vacuum from said vacuum source
to bind molecules of interest to said chromatography media;
introducing said centrifugal filter unit downstream of said first filter unit;
eluting said molecules of interest from said chromatography media into said centrifugal filter unit;
removing said centrifugal filter unit from said manifold; and
centrifuging said centrifugal filter unit.
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