US 7,544,772 B2
Methods for regulating inflammatory mediators and peptides useful therein
Shuji Takashi, Nagano (Japan); Indu Parikh, Chapel Hill, N.C. (US); Kenneth B. Adler, Raleigh, N.C. (US); Linda D. Martin, Apex, N.C. (US); and Yuehua Li, Pearland, Tex. (US)
Assigned to Biomarck Pharmaceuticals, Ltd., Raleigh, N.C. (US)
Filed on Mar. 06, 2006, as Appl. No. 11/367,449.
Application 11/367449 is a continuation in part of application No. 10/802644, filed on Mar. 17, 2004, abandoned.
Application 10/802644 is a continuation of application No. 10/180753, filed on Jun. 26, 2002, abandoned.
Claims priority of provisional application 60/300933, filed on Jun. 26, 2001.
Prior Publication US 2006/0217307 A1, Sep. 28, 2006
Int. Cl. C07K 14/00 (2006.01); C07K 4/00 (2006.01)
U.S. Cl. 530—326  [530/325; 530/327; 530/328; 530/329] 36 Claims
 
1. A method to reduce the exocytotic MARCKS-related release of at least one inflammatory mediator from at least one inflammatory cell comprising contacting the inflammatory cell, which cell comprises the inflammatory mediator contained within a granule inside the cell, with at least one peptide selected from the group consisting of a MANS peptide and an active fragment thereof, wherein said fragment comprises at least six amino acids, in an effective amount to reduce the release of the inflammatory mediator from the inflammatory cell as compared to the release of the inflammatory mediator from the same type of inflammatory cell that would occur in the absence of the peptide,
wherein said inflammatory mediator is selected from the group consisting of myeloperoxidase (MPO), eosinophil peroxidase (EPO), major basic protein (MBP), lysozyme, granzyme, histamine, proteoglycan, protease, cytokine, defensin, bactericidal permeability-increasing protein (BPI), elastase, cathepsin G, cathepsin B, cathepsin D, beta-D-glucuronidase, alpha-mannosidase, phospholipase A2, chondroitin-4-sulphate, proteinase 3, lactoferrin, collagenase, complement activator, complement receptor, N-formylmethionyl-leucyl-phenylalanine (FMLP) receptor, laminin receptor, cytochrome b558, monocyte-chemotactic factor, histaminase, vitamin B12 binding protein, gelatinase, plasminogen activator, and a combination thereof.