| US 7,544,510 B2 | ||
| Stem cells of the islets of Langerhans and their use in treating diabetes mellitus | ||
| Joel F. Habener, Newton Centre, Mass. (US); Elizabeth J. Abraham, Andover, Mass. (US); Henryk Zulewski, Binningen (Switzerland); Melissa K. Thomas, Boston, Mass. (US); and Mario Vallejo, Madrid (Spain) | ||
| Assigned to The General Hospital Corporation, Boston, Mass. (US) | ||
| Filed on Apr. 24, 2006, as Appl. No. 11/410,412. | ||
| Application 11/410412 is a continuation of application No. 09/731261, filed on Dec. 06, 2000, abandoned. | ||
| Claims priority of provisional application 60/238880, filed on Oct. 06, 2000. | ||
| Claims priority of provisional application 60/215109, filed on Jun. 28, 2000. | ||
| Claims priority of provisional application 60/169082, filed on Dec. 06, 1999. | ||
| Prior Publication US 2007/0071730 A1, Mar. 29, 2007 | ||
| Int. Cl. C12N 5/00 (2006.01); C12N 5/06 (2006.01) | ||
| U.S. Cl. 435—325 [435/395; 435/402] | 2 Claims |
| 1. A method of isolating a stem cell from a pancreatic islet of Langerhans, comprising the steps of:
(a) removing a pancreatic islet from a donor;
(b) culturing cells from the pancreatic islet under conditions wherein said cultured cells comprise nestin-positive cells
which have migrated from said islet; and
(c) selecting said migrated nestin-positive cells from the culture, wherein said migrated cells from step (b) form a monolayer.
|