| US 7,544,498 B2 | ||
| Aromatic prenyltransferases, nucleic acids encoding same and uses therefor | ||
| Tomohisa Kuzuyama, Tokyo (Japan); Joseph P. Noel, San Diego, Calif. (US); and Stephane P. Richard, Del Mar, Calif. (US) | ||
| Assigned to The Salk Institute for Biological Studies, La Jolla, Calif. (US); and Toudai TLO, Ltd., Tokyo (Japan) | ||
| Filed on Apr. 18, 2008, as Appl. No. 12/106,181. | ||
| Application 12/106181 is a division of application No. 11/342328, filed on Jan. 27, 2006, granted, now 7,361,483. | ||
| Claims priority of provisional application 60/648046, filed on Jan. 28, 2005. | ||
| Prior Publication US 2008/0274478 A1, Nov. 06, 2008 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. G01N 33/573 (2006.01); C12N 9/10 (2006.01); C12P 1/00 (2006.01) | ||
| U.S. Cl. 435—193 [435/41; 435/7.4] | 7 Claims |
| 1. A method of screening potential binding agent(s) to determine those which bind aromatic prenyltransferase(s) wherein said
aromatic prenyltransferase(s) have a beta/alpha barrel structure and at least 95% sequence identity with the amino acid sequence
set forth in SEQ ID NO:2, said method comprising:
contacting said potential binding agent(s) with said aromatic prenyltransferase(s) in the presence of an aromatic prenyltransferase
substrate; and
determining the ability of said potential binding agent to compete with said aromatic prenyltransferase substrate binding
to said aromatic prenyltransferase(s), wherein the potential binding agent has been modeled to fit spatially into an aromatic
prenyltransferase interaction site defined by a plurality of atomic coordinates.
|