US 7,544,497 B2
	Compositions for manipulating the lifespan and stress response of cells and organisms
David A. Sinclair, Chestnut Hill, Mass. (US); Konrad T. Howitz, Allentown, Pa. (US); Robert E. Zipkin, Wynnewood, Pa. (US); Kevin J. Bitterman, Boston, Mass. (US); and Dudley W. Lamming, Somerville, Mass. (US)
Assigned to President and Fellows of Harvard College, Cambridge, Mass. (US); and Biomol International LP, Plymouth Meeting, Pa. (US)
Filed on Jul. 01, 2004, as Appl. No. 10/884,879.
Claims priority of provisional application 60/483949, filed on Jul. 01, 2003.
Claims priority of provisional application 60/532158, filed on Dec. 23, 2003.
Prior Publication US 2005/0136537 A1, Jun. 23, 2005
Int. Cl. C12N 9/00 (2006.01)

U.S. Cl. 435—183  [435/366]

12 Claims

1. A method for increasing the deacetylase activity of SIRT1in a eukaryotic cell, comprising contacting a eukaryotic cell with a non-naturally occurring agent that binds SIRT1, reduces the Km of SIRT1 for substrate and thereby increases the deacetylase activity of SIRT1.