US 7,544,484 B2
	Nucleic acids encoding membrane-binding proteins and methods of using same
Yoshihiro Ohmiya, Ikeda (Japan); Emiko Ashitaka, Moriguchi (Japan); and Seiji Ito, Moriguchi (Japan)
Assigned to National Institute of Advanced Industrial Science and Technology, Tokyo (Japan)
Appl. No. 10/526,569 PCT Filed Sep. 04, 2003, PCT No. PCT/JP03/11285 § 371(c)(1), (2), (4) Date Mar. 04, 2005, PCT Pub. No. WO2004/022600, PCT Pub. Date Mar. 18, 2004.
Claims priority of application No. 2002-261229 (JP), filed on Sep. 06, 2002; and application No. 2002-357407 (JP), filed on Dec. 10, 2002.
Prior Publication US 2007/0015229 A1, Jan. 18, 2007
Int. Cl. C12P 21/06 (2006.01)

U.S. Cl. 435—69.1  [435/6; 435/7.1; 435/320; 435/325; 435/252]

5 Claims

1. An isolated polynucleotide comprising the sequence of SEQ ID NO: 1, said polynucleotide encodes a secretory or membrane-binding chimeric protein, wherein the chimeric protein is composed of Vargula luciferase and Yellow fluorescent protein (YFP).