| US 7,544,474 B2 | ||
| Method for detection of multiple test materials in a sample | ||
| Hai Xing Chen, Toronto (Canada) | ||
| Assigned to ACGT Medico Inc., Toronto (Canada) | ||
| Filed on Mar. 23, 2007, as Appl. No. 11/728,238. | ||
| Application 11/728238 is a continuation of application No. 10/035861, filed on Dec. 26, 2001, granted, now 7,195,867. | ||
| Application 10/035861 is a continuation in part of application No. 09/326297, filed on Jun. 04, 1999, granted, now 6,337,214. | ||
| Application 09/326297 is a continuation in part of application No. 09/093532, filed on Jun. 08, 1998, granted, now 6,174,733. | ||
| Prior Publication US 2007/0172884 A1, Jul. 26, 2007 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12Q 1/68 (2006.01); C07H 21/04 (2006.01) | ||
| U.S. Cl. 435—6 [536/24.31; 536/24.32] | 25 Claims |
| 1. A method for sequentially detecting multiple test materials in a test sample comprises the steps in sequence of:
(a) adding a test sample into a test column, said test column having at least two different target capture materials on a
single test snare without separation, a first target capture material being specific to a first test material in said test
sample and a second target capture material being specific to a second test material in said test sample; and wherein said
first test material binds to said first target capture material to form a bound first test material on said test snare and
said second test material binds to said second target capture material to form a bound second test material on said test snare;
(b) washing said test column to remove unbound test materials;
(c) adding a first probe to attach specifically to said bound first test material on said test snare, said first probe having
thereon a first chemical label;
(d) washing said test column to remove unbound first probe;
(e) detecting signals generated by said first chemical label on said test snare for determining the presence of said first
test material;
(f) adding a second probe to attach specifically to said bound second test material on said test snare in step (c), said second
probe having thereon a second chemical label;
(g) washing said test column to remove unbound second probe; and
(h) detecting signals generated by said second chemical label on said test snare for determining the presence of said second
test material.
|