US 7,381,471 B2
Hybrid polymers for functional tuning of microfluidic device surfaces
Brian H. Augustine, Bridgewater, Va. (US); James P. Landers, Charlottesville, Va. (US); Jerome P. Ferrance, Charlottesville, Va. (US); Joy M. Polefrone, Charlottesville, Va. (US); and W. Christopher Hughes, Harrisonburg, Va. (US)
Assigned to University of Virginia Patent Foundation, Charlottesville, Va. (US); and James Madison University, Harrisonburg, Va. (US)
Appl. No. 10/520,763
PCT Filed Jul. 15, 2003, PCT No. PCT/US03/22162
§ 371(c)(1), (2), (4) Date Jan. 10, 2005,
PCT Pub. No. WO2004/007582, PCT Pub. Date Jan. 22, 2004.
Claims priority of provisional application 60/399633, filed on Jul. 30, 2002.
Claims priority of provisional application 60/396153, filed on Jul. 15, 2002.
Prior Publication US 2006/0057402 A1, Mar. 16, 2006
Int. Cl. B32B 25/20 (2006.01)
U.S. Cl. 428—447  [428/451] 19 Claims
 
1. A method of preparing a microfluidic device said method comprising the steps of
providing a silica based coverplate, wherein said coverplate comprises a first and second port, and a base, wherein said base comprises a microchannel;
coating a surface of the silica based coverplate or the microchannel bearing surface of the base with a first composition comprising a polymer produced by polymerizing a mixture comprising methylmethacrylate, and a compound of the general structure:

OG Complex Work Unit Drawing
wherein R1, R2, R3, R4, R5, R6, and R7 are independently selected from the group consisting of C1-C6 alkyl, C2-C6 alkenyl, C2-C6 alkynyl, C3-C8 cycloalkyl, and C5-C6 aryl;
contacting the silica based coverplate with the microchannel bearing surface of the base, for a predetermined length of time, in an orientation that places the first and second ports of the coverplate in fluid communication with the microchannel and places the coated surface between the coverplate and the base;
heating the contacted surfaces to a temperature ranging from about 100° C. to about 200° C.; and
contacting the inner surface of the microchannel with a second composition comprising a polymer produced by polymerizing a mixture comprising methylmethacrylate, and a compound of the general structure:

OG Complex Work Unit Drawing
wherein R11, R12, R13, R14, R15, R16, and R17 are independently selected from the group consisting of C1-C18 alkyl, C2-C18 alkenyl, C2-C18 alkynyl, C1-C8 alkylcarboxyl, C1-C8 alkyl(NR20R21R22)+, C1-C8 alkyl(NR21R22), C1-C8 alkyl(NHR22), C1-C8 alkylsulfate, C3-C8 cycloalkyl, and C5-C6 aryl, wherein R20, R21 and R22 are independently selected from the group consisting of C1-C4 alkyl.