US 7,541,189 B2
Transcription activation complex and utilization thereof
Norihisa Ohe, Nara (Japan)
Assigned to Sumitomo Chemical Company, Limited, Tokyo (Japan)
Appl. No. 10/465,914
PCT Filed Dec. 17, 2001, PCT No. PCT/JP01/11062
§ 371(c)(1), (2), (4) Date Jun. 27, 2003,
PCT Pub. No. WO02/053735, PCT Pub. Date Jul. 11, 2002.
Claims priority of application No. 2000-398548 (JP), filed on Dec. 27, 2000; and application No. 2001-077740 (JP), filed on Mar. 19, 2001.
Prior Publication US 2006/0216705 A1, Sep. 28, 2006
Int. Cl. C12N 15/00 (2006.01); C12N 15/63 (2006.01); C07K 14/00 (2006.01); A61K 48/00 (2006.01)
U.S. Cl. 435—455  [530/350; 435/325] 6 Claims
 
1. An in vitro complex of a transcription coupling factor of any one of ARNT 1 to 3 and a transcription regulatory factor comprising any of the amino acid sequences shown below, which is a transcription activating complex that binds to a DNA region (5′-ACGTG-3′, SEQ ID NO: 16) in competition with a transcription inhibiting complex of a Sim2 as a transcription regulatory factor and a transcription coupling factor of any one of ARNT 1 to 3 and which promotes transcription of a gene located downstream of the DNA region, wherein the amino acid sequences are:
(a) the amino acid sequence represented by any one of SEQ ID NOs: 1 to 3,
(b) an amino acid sequence of a protein comprising an amino acid sequence exhibiting an amino acid identity of 90% or more to the amino acid sequence represented by any of SEQ ID NOs: 1 to 3 and regulates transcription of a gene located downstream of a DNA region comprising the nucleotide sequence of SEQ ID NO:16.