| US 7,541,186 B2 | ||
| Method of generating human retinal progenitors from embryonic stem cells | ||
| Thomas Reh, Seattle, Wash. (US); and Deepak Lamba, Seattle, Wash. (US) | ||
| Assigned to University of Washington, Seattle, Wash. (US) | ||
| Filed on Feb. 22, 2006, as Appl. No. 11/361,051. | ||
| Prior Publication US 2007/0196919 A1, Aug. 23, 2007 | ||
| Int. Cl. C12N 5/00 (2006.01) | ||
| U.S. Cl. 435—377 [435/325; 435/375] | 8 Claims |
| 1. A method for in vitro differentiation of human retinal progenitor cells from human embryonic stem cells, the method comprising:
culturing said human embryonic stem cells to embryoid bodies in a conditioning medium comprising at least 1 ng/ml of insulin-like
growth factor 1 (IGF-1), at least 1 ng/ml Dkk-1 and at least 1 ng/ml Noggin for 1 to 7 days in the absence of basic fibroblast
growth factor (FGF2) activity; then
culturing the resulting embryoid bodies in retinal differentiation medium comprising at least 1 ng/ml of insulin-like growth
factor 1 (IGF-1), at least 1 ng/ml Dkk-1, at least 1 ng/ml Noggin and at least 5 ng/ml FGF-2 for at least one week,
thereby yielding human retinal progenitor cells.
|