| US 7,541,185 B2 | ||
| Methods for identifying factors for differentiating definitive endoderm | ||
| Kevin Allen D'Amour, San Diego, Calif. (US); Alan D. Agulnick, San Marcos, Calif. (US); Susan Eliazer, San Diego, Calif. (US); and Emmanuel E. Baetge, Encinitas, Calif. (US) | ||
| Assigned to Cythera, Inc., San Diego, Calif. (US) | ||
| Filed on Jun. 23, 2005, as Appl. No. 11/165,305. | ||
| Application 11/165305 is a continuation in part of application No. 11/115868, filed on Apr. 26, 2005, abandoned. | ||
| Application 11/165305 is a continuation in part of application No. 11/165305, filed on Jun. 23, 2005. | ||
| Application 11/165305 is a continuation in part of application No. 11/021618, filed on Dec. 23, 2004, granted, now 7,510,876. | ||
| Claims priority of provisional application 60/566293, filed on Apr. 27, 2004. | ||
| Claims priority of provisional application 60/587942, filed on Jul. 14, 2004. | ||
| Claims priority of provisional application 60/586566, filed on Jul. 09, 2004. | ||
| Claims priority of provisional application 60/532004, filed on Dec. 23, 2003. | ||
| Prior Publication US 2006/0003313 A1, Jan. 05, 2006 | ||
| Int. Cl. C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12Q 1/00 (2006.01) | ||
| U.S. Cl. 435—377 [435/4] | 31 Claims |
| 1. A method of identifying a factor that promotes the differentiation of human definitive endoderm cells, said method comprising
the steps of:
obtaining a human cell population comprising human definitive endoderm cells, wherein said human definitive endoderm cells
comprise at least 10% of the human cells in said cell population;
providing a candidate differentiation factor to said cell population;
determining expression of a marker of a cell differentiated from definitive endoderm in said cell population at a first time
point;
determining expression of the same marker in said cell population at a second time point, wherein said second time point is
subsequent to said first time point and wherein said second time point is subsequent to providing said cell population with
said candidate differentiation factor; and
determining if expression of the marker in said cell population at said second time point is increased as compared to the
expression of the marker in said definitive endoderm cell population at said first time point, wherein an increase in expression
of said marker in said cell population indicates that said candidate differentiation factor promotes the differentiation of
said human definitive endoderm cells.
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