| US 7,541,184 B2 | ||
| Activation and expansion of cells | ||
| Ronald J. Berenson, Mercer Island, Wash. (US); Che Law, Shoreline, Wash. (US); Mark Bonyhadi, Issaquah, Wash. (US); Narinder Saund, Seattle, Wash. (US); Stewart Craig, Issaquah, Wash. (US); Alan Hardwick, Seattle, Wash. (US); Dale Kalamasz, Redmond, Wash. (US); David McMillen, Seattle, Wash. (US); and Harjinder Singh Chana, Issaquah, Wash. (US) | ||
| Assigned to Invitrogen Corporation, Carlsbad, Calif. (US) | ||
| Filed on Jan. 20, 2004, as Appl. No. 10/762,210. | ||
| Application 10/762210 is a continuation in part of application No. 10/350305, filed on Jan. 22, 2003. | ||
| Application 10/350305 is a continuation in part of application No. 10/187467, filed on Jun. 28, 2002. | ||
| Application 10/187467 is a continuation in part of application No. 10/133236, filed on Apr. 26, 2002, granted, now 6,867,041. | ||
| Application 10/133236 is a continuation in part of application No. 09/960264, filed on Sep. 20, 2001, granted, now 6,797,514. | ||
| Application 09/960264 is a continuation in part of application No. 09/794230, filed on Feb. 26, 2001, granted, now 6,905,874. | ||
| Claims priority of provisional application 60/249902, filed on Nov. 17, 2000. | ||
| Claims priority of provisional application 60/184788, filed on Feb. 24, 2000. | ||
| Prior Publication US 2004/0241162 A1, Dec. 02, 2004 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12N 5/00 (2006.01) | ||
| U.S. Cl. 435—375 [435/325; 435/372.3] | 2 Claims |
| 1. A method for activating and expanding a population of regulatory T cells by simultaneous T cell concentration and cell
surface moiety ligation, comprising:
a. contacting a population of cells wherein at least a portion thereof comprises regulatory T cells with a surface, wherein
said surface is a surface of a paramagnetic particle and wherein said surface has attached thereto a first agent that ligates
a first T cell surface moiety of a regulatory T cell, and the same surface has attached thereto a second agent that ligates
a second moiety of said regulatory T cell; and
b. applying a magnetic force that predominantly drives T cell concentration and T cell surface moiety ligation;
thereby inducing activation and expansion of said regulatory T cell.
|