| US 7,541,173 B2 | ||
| Solvent tolerant microorganisms and methods of isolation | ||
| Michael G. Bramucci, Boothwyn, Pa. (US); Vasantha Nagarajan, Wilmington, Del. (US); Natalia Sedkova, Cherry Hill, N.J. (US); and Manjari Singh, West Chester, Pa. (US) | ||
| Assigned to E.I. du Pont de Nemours and Company, Wilmington, Del. (US) | ||
| Filed on Jun. 12, 2007, as Appl. No. 11/761,497. | ||
| Claims priority of provisional application 60/813779, filed on Jun. 15, 2006. | ||
| Prior Publication US 2008/0124774 A1, May 29, 2008 | ||
| Int. Cl. C12N 1/20 (2006.01); C12N 1/21 (2006.01); C12P 7/16 (2006.01) | ||
| U.S. Cl. 435—252.3 [435/148; 435/160; 435/252.2; 435/252.31; 435/252.34; 435/252.4; 435/252.9] | 28 Claims |
| 1. A method for the isolation of a butanol tolerant Lactobacillus microorganism comprising:
a) providing a microbial sample comprising a microbial consortium;
b) contacting the microbial consortium with a growth medium comprising a fermentable carbon source until the members of the
microbial consortium are growing;
c) contacting the growing microbial consortium of step (b) with butanol; and
d) isolating the viable members of step (c) wherein a butanol tolerant microorganism is isolated wherein the viable members of step (d) are tolerant to at least one of the following:i) 2.5% w/v 1-butanol when grown on a solid medium at 37.degree. C.;ii) 3.9% w/v 2-butanol when grown on a solid medium at 37.degree. C.;iii) 2.7% w/v isobutanol when grown on a solid medium at 37.degree. C.;iv) 5.0% w/v 2-butanone when grown on a solid medium at 37.degree. C.; orv) 9.0% w/v ethanol I when grown on a solid medium at 37.degree. C.
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