| US 7,541,164 B2 | ||
| Mammalian cell culture processes for protein production | ||
| Bernhard M. Schilling, Syracuse, N.Y. (US); Linda Matlock, Parish, N.Y. (US); Stephen G. Zegarelli, North Syracuse, N.Y. (US); William V. Burnett, Fayetteville, N.Y. (US); Christoph E. Joosten, Manlius, N.Y. (US); Jonathan D. Basch, East Syracuse, N.Y. (US); Sivakesava Sakhamuri, Syracuse, N.Y. (US); and Steven S. Lee, Manlius, N.Y. (US) | ||
| Assigned to Bristol-Myers Squibb Company, Princeton, N.J. (US) | ||
| Filed on Dec. 18, 2003, as Appl. No. 10/742,564. | ||
| Claims priority of provisional application 60/436101, filed on Dec. 23, 2002. | ||
| Prior Publication US 2005/0019859 A1, Jan. 27, 2005 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12N 5/06 (2006.01); A61K 39/395 (2006.01); A61K 38/00 (2006.01) | ||
| U.S. Cl. 435—70.1 [435/41; 424/143.1; 424/185.1; 530/388.22; 530/387.3] | 18 Claims |
| 1. A cell culturing process comprising:
a) culturing host cells which produce soluble cytotoxic T-lymphocyte antigen-4 (CTLA4) molecules at a first temperature of
37° C. under cell culture conditions and for a time period that allows for cell growth;
b) then culturing the cells at a second temperature of 34° C;
c) then culturing the cells at a third temperature of 32° C. and
d) then culturing the cells at a fourth temperature of 30° C. and wherein the cells are cultured at the second temperature starting about day 5 to day 7 of the culture, and wherein there are
approximately four days between the start of the second temperature and the start of the third temperature and wherein the
fourth temperature starts on or about two weeks from the start of the culture until the end of the culturing process.
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