| US 7,541,145 B2 | ||
| Cleavage of nucleic acids | ||
| James R. Prudent, Madison, Wis. (US); Jeff G. Hall, Madison, Wis. (US); Victor I. Lyamichev, Madison, Wis. (US); Mary Ann D. Brow, Madison, Wis. (US); and James E. Dahlberg, Madison, Wis. (US) | ||
| Assigned to Third Wave Technologies, Inc., Madison, Wis. (US) | ||
| Filed on Jul. 15, 2005, as Appl. No. 11/183,078. | ||
| Application 09/350309 is a division of application No. 08/756386, filed on Nov. 26, 1996, granted, now 5,985,557. | ||
| Application 11/183078 is a continuation of application No. 09/982667, filed on Oct. 18, 2001, granted, now 7,011,944. | ||
| Application 09/982667 is a continuation of application No. 09/350309, filed on Jul. 09, 1999, granted, now 6,348,314. | ||
| Application 08/756386 is a continuation in part of application No. 08/682853, filed on Jul. 12, 1996, granted, now 6,001,567. | ||
| Application 08/682853 is a continuation in part of application No. 08/599491, filed on Jan. 24, 1996, granted, now 5,846,717. | ||
| Prior Publication US 2006/0177835 A1, Aug. 10, 2006 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C12Q 1/68 (2006.01); C12P 19/34 (2006.01) | ||
| U.S. Cl. 435—6 [435/91.2] | 27 Claims |
| 1. A method for detecting a target nucleic acid comprising:
exposing a composition comprising a purified thermostable FEN-1 endonuclease from an archaebacterial species selected from
the group consisting of Pyrococcus furiosus and Methanococcus jannaschii and a purified nucleic acid molecule, wherein said purified nucleic acid molecule is capable of hybridizing to a target nucleic
acid to form a FEN-1 endonuclease cleavage structure, to a sample suspected of containing said target nucleic acid;
forming a FEN-1 endonuclease cleavage structure; and
cleaving said FEN-1 endonuclease cleavage structure with said purified thermostable FEN-1 endonuclease, such that said target
nucleic acid is detected.
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