| US 7,541,039 B2 | ||
| Immunization with bacterial ghost-based vaccines | ||
| Cornelis Johannes Leenhouts, Haren (Netherlands); Ranjan Ramasamy, Colombo (Sri Lanka); Anton Steen, Groningen (Netherlands); Jan Kok, Groningen (Netherlands); Girbe Buist, Sappemeer (Netherlands); and Oscar Paul Kuipers, Groningen (Netherlands) | ||
| Assigned to Applied NanoSystems, B.V., Groningen (Netherlands) | ||
| Filed on Dec. 09, 2005, as Appl. No. 11/299,304. | ||
| Application 11/299304 is a division of application No. 10/321857, filed on Dec. 16, 2002, granted, now 7,067,639. | ||
| Application 10/321857 is a continuation of application No. PCT/NL02/00383, filed on Jun. 11, 2002. | ||
| Claims priority of application No. 01202239 (EP), filed on Jun. 11, 2001. | ||
| Prior Publication US 2006/0115491 A1, Jun. 01, 2006 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. A61K 39/02 (2006.01); A61K 39/00 (2006.01); A61K 39/002 (2006.01); A61K 39/385 (2006.01); A61K 39/116 (2006.01); A61K 39/09 (2006.01); A61K 45/00 (2006.01); A61K 39/07 (2006.01); A61K 39/04 (2006.01); A61K 39/015 (2006.01); C12N 1/00 (2006.01); C12N 1/06 (2006.01) | ||
| U.S. Cl. 424—234.1 [424/193.1; 424/190.1; 424/191.1; 424/203.1; 424/244.1; 424/266.1; 424/265.1; 424/279.1; 424/278.1; 424/282.1; 424/246.1; 424/248.1; 424/268.1; 424/272.1; 435/259; 435/243] | 14 Claims |
| 1. A method for immunizing a subject, said method comprising:
administering a vaccine to a mucosal membrane of the subject, said vaccine comprising:
a proteinaceous substance comprising an immunogenic determinant attached to cell wall material from a Gram positive bacterium,
wherein said cell wall material consists essentially of spherical peptidoglycan microparticles, the microparticles being obtainable
by treating a cell wall with an acid solution, thus removing a cell wall component selected from the group consisting of a
protein, lipotechoic, a carbohydrate, and combinations thereof from said cell wall material;
so as to immunize the subject.
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