| US 7,537,756 B2 | ||
| Stem cells and their use in transplantation | ||
| Joel E. Habener, Newton Centre, Mass. (US); Hendrik Zulewski, Basel (Switzerland); Elizabeth J. Abraham, Quincy, Mass. (US); Mario Vallejo, Madrid (Spain); Denise L. Faustman, Weston, Mass. (US); and Melissa K. Thomas, Boston, Mass. (US) | ||
| Assigned to The General Hospital Corporation, Boston, Mass. (US) | ||
| Filed on May 19, 2005, as Appl. No. 11/132,687. | ||
| Application 11/132687 is a division of application No. 10/136891, filed on May 02, 2002, granted, now 6,923,959. | ||
| Application 10/136891 is a continuation in part of application No. 09/731255, filed on Dec. 06, 2000, granted, now 6,866,843. | ||
| Claims priority of provisional application 60/238880, filed on Oct. 06, 2000. | ||
| Claims priority of provisional application 60/215109, filed on Jun. 28, 2000. | ||
| Claims priority of provisional application 60/169082, filed on Dec. 06, 1999. | ||
| Prior Publication US 2006/0062769 A1, Mar. 23, 2006 | ||
| Int. Cl. A61K 35/12 (2006.01); C12N 5/00 (2006.01) | ||
| U.S. Cl. 424—93.7 [424/93.1; 424/520; 435/1.1; 435/4; 435/325] | 17 Claims |
| 1. A method of treating a patient with diabetes mellitus, comprising the steps of:
(a) isolating a nestin-positive pancreatic stem cell from a pancreatic islet of a donor;
(b) culturing the stem cell ex vivo to produce a progenitor cell; and
(c) transferring the progenitor cell into the patient, wherein the progenitor cell differentiates into an insulin-producing
beta cell,
wherein the patient does not serve as the donor for said stem cells of step (a);
thereby treating said patient with diabetes mellitus.
|