	US 7,531,726 B2
	Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading
Selena Chan, San Jose, Calif. (US); Xing Su, Cupertino, Calif. (US); and Mineo Yamakawa, Campbell, Calif. (US)
Assigned to Intel Corporation, Santa Clara (US)
Filed on Mar. 11, 2005, as Appl. No. 11/77,577.
Application 11/077577 is a division of application No. 10/251152, filed on Sep. 20, 2002.
Prior Publication US 2005/0208554 A1, Sep. 22, 2005
Int. Cl. C07H 21/02 (2006.01); C07H 21/04 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 3/00 (2006.01)

U.S. Cl. 977—704 [977/728; 977/734; 977/742; 977/773; 977/774; 536/23.1; 536/24.3; 536/24.33; 435/283.1; 435/287.1; 435/287.2]

6 Claims

1. A system for nucleic acid sequencing comprising:

a) a scanning probe microscope;

b) a substrate;

c) at least one target molecule immobilized on the substrate, wherein the target molecule is a nucleic acid;

d) one or more coded oligonucleotide probes ligated to the nucleic acid, wherein each of the coded oligonucleotide probes is attached to at least one nanobarcode; and

e) a surface, wherein the coded oligonucleotide probes are attached to the surface after the coded oligonucleotide probes are separated from the nucleic acid; and

f) a free flow electrophoresis device, wherein the free flow electrophoresis device is configured to align the separated coded oligonucleotide probes on the surface wherein the surface comprises alternating bands of conductive and non-conductive materials such that in the presence of an alternating current electrical field, the free flow electrophoresis device is configured to align the separated coded oligonucleotide probes on the surface.