| US 7,531,715 B1 | ||
| Double nuclear transfer method and results thereof | ||
| Keith H. S. Campbell, Leicestershire (United Kingdom) | ||
| Assigned to PPL Therapeutics (Scotland), Edinburgh (United Kingdom) | ||
| Appl. No. 9/600,130 PCT Filed Jan. 13, 2000, PCT No. PCT/GB00/00086 § 371(c)(1), (2), (4) Date May 14, 2001, PCT Pub. No. WO00/42174, PCT Pub. Date Jul. 20, 2000. |
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| Claims priority of application No. 9900734.6 (GB), filed on Jan. 13, 1999. | ||
| Int. Cl. C12N 15/00 (2006.01); C12N 5/00 (2006.01); C12N 5/02 (2006.01); G01N 33/00 (2006.01); A01K 67/027 (2006.01) | ||
| U.S. Cl. 800—24 [800/3; 800/14; 800/21; 435/325] | 35 Claims |
| 1. A method of reconstituting a non-primate mammalian embryo, comprising
(i) transferring a non-primate mammalian donor nucleus from a diploid somatic cell into a first recipient oocyte;
(ii) removing the donor nucleus from the first recipient oocyte;
(iii) providing a second recipient oocyte or enucleated fertilized zygote; and
(iv) transferring the donor nucleus from the first recipient oocyte into the second recipient oocyte or the enucleated fertilized
zygote to obtain a reconstituted non-primate mammalian embryo, wherein the second recipient oocyte is activated prior to,
concomitant with or following transfer of the donor nucleus.
|