| US 7,531,626 B2 | ||
| Tenebrio antifreeze proteins | ||
| Laurie A. Graham, Kingston (Canada); Yih-Cherng Liou, Kingston (Canada); Virginia K. Walker, Sydenham (Canada); and Peter L. Davies, Kingston (Canada) | ||
| Assigned to Queen's University at Kingston, Kingston, Ontario (Canada) | ||
| Filed on Jun. 04, 2004, as Appl. No. 10/861,858. | ||
| Application 10/861858 is a division of application No. 10/032658, filed on Nov. 08, 2001, granted, now 6,747,130. | ||
| Application 10/032658 is a division of application No. 08/882907, filed on Jun. 26, 1997, granted, now 6,392,024. | ||
| Prior Publication US 2006/0116506 A1, Jun. 01, 2006 | ||
| Int. Cl. C07K 14/00 (2006.01); C07H 21/00 (2006.01); C12P 21/00 (2006.01); C12N 5/10 (2006.01); C12N 1/21 (2006.01); C12N 1/15 (2006.01); C12N 5/06 (2006.01); C12N 1/16 (2006.01) | ||
| U.S. Cl. 530—350 [435/69.1; 435/325; 435/410; 435/252.3; 435/254.11; 435/255.1; 435/348; 435/252.1; 536/23.1] | 19 Claims |
| 1. An isolated or recombinantly expressed antifreeze protein, said protein comprising the following:
(i) a calculated molecular weight of between 7 and 13 kDa;
(ii) a thermal hysteresis activity of greater than 1.5° C. at a concentration of about 1 mg/mL;
(iii) the N-terminal amino acid motif set forth in SEQ ID NO:3;
(iv) at least 70% amino acid sequence identity to the antifreeze protein of SEQ ID NO:15 (YL-4); and
(v) at least 4 repeats of the 12 contiguous amino acid motif set forth in SEQ ID NO:1.
|