US 7,531,316 B2
	High throughput assay of Lp-PLA2 activity
Yun-Fu Hu, Research Triangle Park, N.C. (US); and George T. Walker, Research Triangle Park, N.C. (US)
Assigned to Glaxo Group Limited, Greenford, Middlesex (United Kingdom)
Appl. No. 10/557,540 PCT Filed May 27, 2004, PCT No. PCT/US2004/016716 § 371(c)(1), (2), (4) Date Nov. 21, 2005, PCT Pub. No. WO2005/001416, PCT Pub. Date Jan. 06, 2005.
Claims priority of provisional application 60/473777, filed on May 28, 2003.
Prior Publication US 2007/0065892 A1, Mar. 22, 2007
Int. Cl. C12Q 1/34 (2006.01)

U.S. Cl. 435—18  [435/198]

53 Claims

1. A method for determining an Lp-PLA2 enzyme activity in a plurality of samples comprising the steps of preparing a solution comprising labeled PAF; contacting each of a plurality of tissue samples with the solution of the preparing step and with a sequester molecule of PAF, wherein said sequester molecule is exogenous BSA, to form a PAF-sequester molecule complex; removing said PAF-sequester molecule complex; and detecting an Lp-PLA2 activity by measuring the amount of PAF cleaved by said Lp-PLA2 enzyme wherein said amount of cleaved PAF determines Lp-PLA2 activity.