| US 7,531,308 B2 | ||
| Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography | ||
| Kevin Bernard Ray, Ballwin, Mo. (US); Carol Ann Kreader, Kirkwood, Mo. (US); Fuqiang Chen, Saint Louis, Mo. (US); and David Eric Cutter, High Ridge, Mo. (US) | ||
| Assigned to Sigma-Aldrich Co., St. Louis, Mo. (US) | ||
| Filed on Apr. 18, 2005, as Appl. No. 11/108,317. | ||
| Claims priority of provisional application 60/565026, filed on Apr. 23, 2004. | ||
| Prior Publication US 2005/0245733 A1, Nov. 03, 2005 | ||
| Int. Cl. C12Q 1/68 (2006.01); C12N 1/06 (2006.01); C12N 15/00 (2006.01); C12P 19/34 (2006.01) | ||
| U.S. Cl. 435—6 [435/259; 435/320.1] | 16 Claims |
| 1. A method for reducing endotoxin levels in a nucleic acid solution comprising:
a) contacting a nucleic acid solution with a carbohydrate non-ionic detergent selected from the group consisting of alkyl
thioglucosides, alkyl glucosides and alkyl maltosides;
b) contacting the solution resulting from step a) to an inorganic binding matrix;
c) washing the binding matrix resulting from step b) to obtain a nucleic acid composition having an endotoxin content of less
than 100 EU/mg.
|