| US 7,531,163 B2 | ||
| Enrichment methods for the detection of pathogens and other microbes | ||
| Mansour Samadpour, Seattle, Wash. (US) | ||
| Assigned to Institute for Environmental Health, Inc., Lake Forest Park, Wash. (US) | ||
| Filed on May 17, 2004, as Appl. No. 10/848,253. | ||
| Claims priority of provisional application 60/470975, filed on May 16, 2003. | ||
| Prior Publication US 2004/0241644 A1, Dec. 02, 2004 | ||
| Int. Cl. A61K 49/00 (2006.01); C12Q 1/00 (2006.01); G01N 33/53 (2006.01) | ||
| U.S. Cl. 424—9.1 [424/130.1; 424/163.1; 424/164.1; 424/184.1; 424/234.1; 435/4; 435/7.2; 435/243] | 23 Claims |
| 1. A method for detection of a particular pathogen or other microbe, comprising:
obtaining a test sample at a first location, the sample being solid or semi-solid;
diluting, at the first location, the sample with liquid enrichment medium at a ratio of sample to diluent of between about
1:0.1 to about 1:2 (wt./vol.), or lesser dilution;
incubating the diluted sample at an optimal temperature for the particular pathogen or other microbe to be detected in an
incubator during transit of the diluted sample to a second remote location that is not the site of sample collection; and
determining, at the remote test location, by assaying the diluted incubated test sample, or a portion thereof, with an assay
suitable to detect the particular pathogen or other microbe, whether the sample is contaminated with the particular pathogen
or other microbe.
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