US 5,955,340 C1 (6812th)
	MODIFIED SUBTILISINS HAVING AMINO ACID ALTERATIONS
Richard Ray Bott, Burlingame, Calif.; Robert Mark Caldwell, San Francisco, Calif.; Brian C. Cunningham, Piedmont, Calif.; David Aaron Estell, Mountain View, Calif.; Scott Douglas Power, San Bruno, Calif., and James Allen Wells, San Mateo, Calif., assignors to Genencor International, Inc., Palo Alto, Calif.
Reexamination Request No. 90/010,039, Oct. 17, 2007.
Reexamination Certificate for Patent 5,955,340, issued Sep. 21, 1999, Appl. No. 485,313, Jun. 7, 1995.
Division of application No. 08/212,291, filed on Mar. 14, 1994, now Pat. No. 5,972,682, which is a continuation of application No. 07/898,382, filed on Jun. 9, 1992, now abandoned, which is a continuation of application No. 07/747,459, filed on Aug. 12, 1991, now abandoned, which is a continuation of application No. 07/540,868, filed on Jun. 14, 1990, now abandoned, which is a continuation of application No. 07/035,652, filed on Apr. 6, 1987, now abandoned, which is a continuation-in-part of application No. 06/858,594, filed on Apr. 30, 1986, now abandoned, which is a continuation-in-part of application No. 06/614,612, filed on May 29, 1984, now Pat. No. 4,760,025, and a continuation-in-part of application No. 06/614,615, filed on May 29, 1984, now abandoned, and a continuation-in-part of application No. 06/614,617, filed on May 29, 1984, now abandoned, and a continuation-in-part of application No. 06/614,491, filed on May 29, 1984, now abandoned.
Int. Cl. C11D 3/338;3/386; C12N 9/52;9/54;9/56;15/10;15/74;15/75 (2006.01)

U.S. Cl. 435—221

AS A RESULT OF REEXAMINATION, IT HAS BEEN DETERMINED THAT:

The patentability of claims 1-97 and 99-102 is confirmed.

Claim 98 is cancelled.

1. A recombinant DNA encoding a subtilisin modified by a substitution of at least one amino acid at a residue position with a different naturally occurring amino acid, said residue position being selected from the group of equivalent amino acid residues of subtilisin naturally produced by Bacillus amyloliquefaciens consisting of Tyr21, Thr22, Ser24, Asp36, Gly46, A1a48, Ser49, Met50, Asn77, Ser87, Lys94, Val95, Leu96, lle107, Gly110, Met124, 1.ys170, Tyr171, Pro172, Asp197, Met199, Ser204, Lys213, His67, 1eu126, Leu135, Gly97, Ser101, Gly102, Trp103, Gly127, Gly128, Pro129, Tyr214 and Gly215, wherein the subtilisin which is modified is selected from the group consisting of subtilisins derived from procaryotes, yeast and fungi.