US 5,700,676 C1 (6810th)
	MODIFIED SUBTILISINS HAVING AMINO ACID ALTERATIONS
Richard Ray Bott, Burlingame, Calif.; Robert Mark Caldwell, San Francisco, Calif.; Brian C. Cunningham, Piedmont, Calif.; David Aaron Estell, Mountain View, Calif.; Scott Douglas Power, San Bruno, Calif., and James Allen Wells, San Mateo, Calif., assignors to Genencor International, Inc., Palo Alto, Calif.
Reexamination Request No. 90/010,038, Oct. 17, 2007.
Reexamination Certificate for Patent 5,700,676, issued Dec. 23, 1997, Appl. No. 486,746, Jun. 7, 1995.
Division of application No. 08/212,291, filed on Mar. 14, 1994, now Pat. No. 5,972,682, which is a continuation of application No. 07/898,382, filed on Jun. 9, 1992, now abandoned, which is a continuation of application No. 07/747,459, filed on Aug. 12, 1991, now abandoned, which is a continuation of application No. 07/540,868, filed on Jun. 14, 1990, now abandoned, which is a continuation of application No. 07/035,652, filed on Apr. 6, 1987, now abandoned, which is a continuation of application No. 06/858,594, filed on Apr. 30, 1986, now abandoned, which is a continuation-in-part of application No. 06/614,612, filed on May 29, 1984, now Pat. No. 4,760,025, and a continuation-in-part of application No. 06/614,615, filed on May 29, 1984, now abandoned, and a continuation-in-part of application No. 06/614,617, filed on May 29, 1984, now abandoned, and a continuation-in-part of application No. 06/614,491, filed on May 29, 1984, now abandoned.
Int. Cl. C11D 3/38;3/386; C12N 15/74;9/52;15/10;15/75;9/54;9/56;7/42 (2006.01)

U.S. Cl. 435—221

AS A RESULT OF REEXAMINATION, IT HAS BEEN DETERMINED THAT:

The patentability of claims 1-22, 43-46 and 50-56 is confirmed.

Claim 57 is cancelled.

Claim 23 is determined to be patentable as amended.

Claims 24-42 and 47-49, dependent on an amended claim, are determined to be patentable.

23. A substantially pure subtilisin modified by a substitution of at least one amino acid at a residue position with a different naturally occurring amino acid, said residue position being selected from the group of equivalent amino acid residues of subtilisin naturally produced by Bacillus amyloliquefaciens consisting of His67, Ile107, Leu 135, Gly97, Ala98, [Asp99]Gly100, Ser101, Gly102, Gln103, Leu126, Gly127, Gly128, Pro129, Lys213, Tyr214, Gly215, Gly153, Asn154, Gly157, Thr158, Ser159, Gly160, Ser161, Ser162, Ser163, Thr164, Val165, Tyr167, Pro168, Lys170, Tyr171 and Pro172 wherein said modified subtilisin has altered substrate specificity as compared to the same subtilisin having the amino acid naturally occurring at the residue position, wherein the subtilisin which is modified is selected from the group consisting of subtilisins derived from procaryotes, yeast and fungi.