| US 7,524,942 B2 | ||
| Labeled nucleotide composition | ||
| Hui Wang, Cupertino, Calif. (US); and Jeffrey R. Sampson, San Francisco, Calif. (US) | ||
| Assigned to Agilent Technologies, Inc., Santa Clara, Calif. (US) | ||
| Filed on Jul. 31, 2006, as Appl. No. 11/497,208. | ||
| Prior Publication US 2008/0124708 A1, May 29, 2008 | ||
| This patent is subject to a terminal disclaimer. | ||
| Int. Cl. C07H 21/04 (2006.01); C07H 19/04 (2006.01); C12Q 1/68 (2006.01); C07H 21/00 (2006.01); C12P 19/34 (2006.01) | ||
| U.S. Cl. 536—23.1 [435/6; 435/91.1; 536/24.3; 536/25.3; 536/26.6] | 20 Claims |
| 1. A composition having the structure (I):
P1-Nus-P2-Lnk-Obs (I) or a salt, conjugate base, tautomer, or ionized form thereof,
wherein:
P1 is a phosphate group,
Nus is a nucleoside moiety comprising a sugar group bound to a purine or pyrimidine base;
P2 is a phosphate group;
Lnk is a linking group having the structure
 wherein m and n are integers independently selected from the range of 1 to about 12, the first broken line indicates the
bond to P2, and the second broken line indicates the bond to Obs; and
Obs is an observable label moiety.
|