| US 7,524,667 B2 | ||
| Mutated 6-phosphogluconate dehydrogenase | ||
| Masato Ikeda, Nagano (Japan); Junko Ohnishi, Nagano (Japan); Satoshi Mitsuhashi, Hofu (Japan); and Keiko Ochiai, Ebina (Japan) | ||
| Assigned to Kyowa Hakko Food Specialties Co., Ltd., Yamaguchi (Japan) | ||
| Appl. No. 10/497,502 PCT Filed Dec. 03, 2002, PCT No. PCT/JP02/12661 § 371(c)(1), (2), (4) Date Jun. 03, 2004, PCT Pub. No. WO03/048351, PCT Pub. Date Jun. 12, 2003. |
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| Claims priority of application No. 2001-368264 (JP), filed on Dec. 03, 2001. | ||
| Prior Publication US 2005/0079586 A1, Apr. 14, 2005 | ||
| Int. Cl. C12N 9/04 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01); C12Q 1/00 (2006.01); C12Q 1/68 (2006.01); C12P 21/04 (2006.01); C07H 21/04 (2006.01) | ||
| U.S. Cl. 435—190 [435/4; 435/6; 435/69.1; 435/71.1; 435/440; 435/252.3; 435/320.1; 536/23.2] | 5 Claims |
| 3. An isolated polypeptide selected from the group consisting of polypeptides according to the following (a) and (b):
(a) an isolated polypeptide having a modified amino acid sequence of 6-phosphogluconate dehydrogenase (hereinafter abbreviated
as GND) isolated from a microorganism belonging to the genus Corynebacterium, said modification consisting of substitution of the amino acid residue at the position corresponding to the 158th amino
acid of the amino acid sequence of SEQ ID NO: 1 by an amino acid residue selected from the group consisting of Ser, Thr, Trp,
Cys, Asn, Gln and Tyr, and having GND activity; and
(b) an isolated polypeptide having a modified amino acid sequence of GND isolated from a microorganism belonging to the genus
Corynebacterium, said modification consisting of substitution of the amino acid residue at the position corresponding to the 158th amino
acid of the amino acid sequence of SEQ ID NO: 1 by an amino acid residue selected from the group consisting of Ser, Thr, Trp,
Cys, Asn, Gln and Tyr and substitution of the amino acid residue at the position corresponding to the 361st amino acid of
the amino acid sequence of SEQ ID NO: 1 by an amino acid residue selected from the group consisting of Phe, Val, Leu, Ile,
Met, Gly, Ala, Pro and Trp, and having GND activity,
wherein the GND isolated from the microorganism belonging to the genus Corynebacterium is GND having the amino acid sequence of SEQ ID NO: 1.
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